Synthesis of adenosine analogues with indole moiety as human adenosine A3 receptor ligands

Adenosine is an endogenous modulator exerting its functions through the activation of four adenosine receptor (AR) subtypes, termed A1, A2A, A2B and A3, which belong to the G-protein-coupled receptor superfamily. The human A3AR (hA3AR) subtype is implicated in several cytoprotective functions. Therefore, hA3AR modulators, and in particular agonists, are sought for their potential application as anti-inflammatory, anti-cancer and cardioprotective agents. Here, we prepared novel adenosine derivatives with indole moiety as hA3AR ligands. According to the biological assay, we found that 2-substituents 11 were critical structural determinants for A3AR ligands (Ki = 111 nM). The observed structure–affinity relationships of this class of ligands were also exhaustively rationalized using the molecular modelling approach. This allows the investigation on the binding mode of the potential compound in the ligand-binding pocket of the human A3 receptor. The results demonstrated that 11 can interact with the ASN250, GLN167, PHE168 and VAL178 through hydrogen bonding, which are shown to be important for ligand–receptor interaction.


Introduction
Adenosine is an endogenous purine nucleoside that modulates many physiological processes, which is composed of a molecule of adenine attached to a ribose sugar molecule (ribofuranose) moiety via a β-N 9 -glycosidic bond [1][2][3]. Adenosine is widely found in nature and plays an important role in biochemical processes, such as energy transfer as adenosine triphosphate (ATP) and adenosine diphosphate (ADP), as well as in signal transduction as cyclic adenosine monophosphate (cAMP). It is also a neuromodulator, believed to play a role in promoting sleep and suppressing arousal. Adenosine also plays a role in the regulation of blood flow to various organs through vasodilation [4][5][6]. Cellular signalling by adenosine occurs through four known adenosine receptor (AR) subtypes (A 1 , A 2A , A 2B and A 3 ) [7]. All AR subtypes are G-protein-coupled receptors. The four receptor subtypes are further classified based on their ability to either stimulate or inhibit adenylate cyclase activity. They have long been considered to be promising therapeutic targets in a wide range of conditions, ranging from cerebral diseases to cancer, including inflammatory and immunological disorders [8,9]. Adenosine contributes in a significant manner to the maintenance of tissue integrity by modulating the immune system. Encouraging results have emerged with AR ligands for the management of several physiological conditions in preclinical and clinical settings [7,10].
Among the four AR subtypes, the A 3 AR, probably the most studied AR subtype, is also ubiquitously expressed [11]. The distribution of A 3 AR is species-dependent, and in humans, this subtype is expressed in the lungs, liver, heart, kidneys and brain [12][13][14]. The widespread distribution in different cells and tissues of the A 3 AR suggests a potential involvement in various pathologies and the possible use as a selective pharmacological target [15]. This subtype of ARs is involved in a variety of important pathophysiological processes, ranging from modulation of cerebral and cardiac ischaemic damage to regulation of immunosuppression and inflammation [16].
The increasing knowledge about A 3 ARs, in particular regarding the molecular biology of this subtype, has provided important evidence to consider this receptor as a novel therapeutic target. In addition, it enables rational design and the development of potent and selective A 3 AR ligands as promising therapeutic options for a variety of diseases [17,18]. Therefore, small molecule modulators targeting the A 3 AR have been sought for their potential application as anti-inflammatory, anti-cancer and cardioprotective agents [19][20][21].
Indoles probably represent one of the most important structural classes in drug discovery [22]. The indole substructure is a basic element for a number of biologically active natural and synthetic products. Indoles are found in a wide range of therapeutically important drugs [23,24]. Over the years, a considerable amount of effort has been made to find indoles with various biological activities and select certain agents for leads in drug discovery research. On account of their potent biological activities, indoles have continued to attract the interest of chemists and biologists alike in drug discovery.
In addition, the scientific community is making intensive efforts to design AR ligands endowed with greater selectivity or to develop innovative compounds acting as receptor modulators. To further explore the importance of indole framework as the basis for AR ligands, we investigate the receptor subdomain that binds the purine moiety by the study of 2-O-alkyl derivatives of 2-chloroadenosine. The present paper reports on the synthesis and binding studies of these compounds as well as of adenosine. We describe the lengths of alkyl group in terms of potency at the A 3 receptor as well as receptor subtype selectivity. Here, we use the conversion of the 2-alkynyl group into flexible 2-O-alkyl between purine and indole moiety.

Synthesis
In an effort to discover new nucleoside analogues as potential A 3 AR ligands, we pursued the 2oxypurine nucleoside using indole alkyl iodides as shown in scheme 1. The synthesis of the 5 -CH 2 OH analogues started from 2-amino-6-chloropurine riboside 1, which was converted into 6-chloro-2hydroxy-9-(2,3,5-tri-O-acetyl-β-D-ribofuranosyl)purine 2, as reported [25]. The reaction of the hydroxyl group at the 2-position of 2 with various indole iodides was conducted in the presence of caesium carbonate to affect compounds 3-8. Simultaneous removal of the acetyl group and amination at the 6-position of 3-8 using ammonium hydroxide solution yielded compounds 9-14.
For synthesizing the key compounds, we should synthesize intermediates for the 2-ether component depicted in schemes 2-4. Scheme 2 illustrates the synthesis of two carbons as linker indole iodides. The indole 2-oxoacetate 16 was accessed by the treatment of 6-bromoindole 15 to oxalyl chloride, followed by a reaction with ethanol. Alkylation of indole nitrogen by CH 3 I and BnBr provided N-alkyl 17a and 17b, respectively. The following exposure to BH 3   corresponding alcohols 23a and 23b. Finally, iodides 25a and 25b were derived from 23a-b via tosylate alcohol, which underwent substitution with iodine. The synthetic strategy for the preparation of substituted indole derivatives is described in scheme 4. The commercially available 4-bromoindole-3-carboxaldehyde 26 was reacted with CH 3 I and BnBr to give rise to N-alkylation 27a-b, respectively. The Wittig reaction of aldehyde provided trans-indole acrylate 28a-b. The following treatment with NaBH 4 and BiCl 3 yielded propionates 29a-b. LiAlH 4 reduction of the carboxylic ester produced intermediates 30a-b followed by treatment with tosyl chloride to afford the corresponding 31a-b, and the tosylate moiety was displaced with good yield with iodine to incorporate the iodo functionality. Detailed synthetic procedures including the yield of reactions and characteristic data can be found in the electronic supplementary material.

Binding affinity studies
The aim of the present study is to expand knowledge of the structure-activity relationships at the A 3 AR and at other subtypes, both in relation to binding affinity and intrinsic efficacy, of adenosine derivatives modified in the 2-position. A screening campaign to discover new scaffolds for A 3 AR inhibition yielded the moderate-potency lead 11 (table 1). Among 2-substituted derivatives, 2-ethers were more potent than the corresponding amines or thioethers [26]. The effect of bromine substitution of the phenyl ring was evaluated. This series of 5 -brom analogue showed a tendency towards increased K i values with A 1 and A 3 ARs, depending on the bulkiness of the bromine atom. Of these analogues, compound 10 was equipotent to 13 with A 1 AR. However, its selectivity to binding A 3 AR was improved. Unexpectedly, 14 was threefold less potent than 13 in binding to A 3 AR and was tolerated by A 2A AR. Although the 5 -bromo derivative 13 was somewhat equipotent to 11 with A 3 AR, its selectivity was reduced.
Interestingly, the 3-indolyl analogue 11 was sevenfold more potent than 12 with A 3 AR. The increased size or steric hindrance of the N-substituent markedly decreases A 3 AR potency. Nevertheless, 12 was less potent than 13 in binding to A 2A AR. The effect of the space of the alkyl chain between the 2-ethers and the indole moiety was tested. Elongation increased the affinity for A 3 AR. Compound 11 showed a fivefold increased potency with A 3 AR, while it was somewhat tolerated by A 2A AR. The affinity of 9 to all three AR subtypes showed low potency compared with that of 13, similar to the results with 10.
However, the corresponding 2-indolyl derivative 13 was more potent than compound 11 in affinity for A 1 and A 2A Rs. The bulkiness of the N-substituent may be related to the increased affinity. Compound 14 was more potent than 12 in binding to all three ARs. Meanwhile, compound 11 displayed a fivefold potency enhancement over 9 with A 3 AR. The N-Bn derivative 10 with potency close to that of 9 was invariant in affinity for A 1 and A 2A Rs.

Molecular docking analysis
Driven by docking of several derivatives with hA 3 R, we performed the molecular modelling studies [27] to explore the binding modes of all six aforementioned compounds as shown in     1 and 2). Detailed information for docking experiment can be found in the electronic supplementary material. In particular, the common interactions for these three compounds were the hydrogen bonding with the amino acid ASN250 of the protein, which is shown to be closely relevant to antagonist interaction [28,29]. Furthermore, the docking (d) the cpd9 is coloured green, the cpd11 is coloured magenta, the cpd13 is coloured cyan, respectively. The surrounding residues interacting with these ligands are also shown and labelled. The meaning of the items on the two-dimensional plot above is listed in the bottom panel.
results showed that the compound cpd11 with the highest K i value has the highest predicted affinity (−9.08 kcal mol −1 , table 2). Figure 2 depicts the binding mode of the compound cpd11 in the ligand-binding pocket of the protein in details. Cpd11 can be well docked into the binding site of interest. In the most potent molecule cpd11, the adenosine core contributed strongly to the binding affinity, which also demonstrates the rationality of the core as a key scaffold for the further chemical modifications. The hydroxyl oxygen of furan ring and the 6-postion N atom of adenyl group interact with the ASN250, GLN167, PHE168 and VAL178 through hydrogen bonding. On the other hand, the high affinity also requires the presence of the 2-O-alkyl-substituted groups for producing the strong hydrophobic interactions.
From these results, we have illustrated the interactions of our newly synthesized 2-O-alkylsubstituted adenosine analogues with a ligand-binding site of hA 3 AR from the molecular modelling point of view. The results also showed the different roles of the substitutions which are strongly linked to the increased or decreased affinity. The exploration for these interactions can provide us the guidance for the future chemical modifications.

Conclusion
In this work, we designed and synthesized a series of 2-O-alkyl-substituted adenosine analogues with indole moiety. The 2-substituents 11 was the most potent among the series, and it was confirmed to be a modulator in a functional assay measuring its capacity to bind receptors in CHO cells expressing the hA 3 A receptor. We found that 2-substituents 11 were critical structural determinant for A 3 AR ligands (K i = 111 nM). The promising compound can be considered a valuable seed for the design and development of new and even more selective and potent compounds. The molecular modelling studies have also been performed to investigate the binding mode of the potential compound in the ligandbinding pocket of human A 3 receptor. Here, this study provides useful foundations for the attainment of a detailed pharmacological and physiological characterization of the adenosine A 3 receptor.
Data accessibility. The detailed experimental synthetic procedures and spectra of the final compounds are provided in the electronic supplementary material.