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laboratory mutt genome sequence: insights into ancestry and physiology of a W303-K6001 cross-platform Saccharomyces cerevisiae The Supplementary data any medium, provided the original work is properly cited. (7 articles) systems biology (22 articles) genetics (11 articles) bioinformatics Articles on similar topics can be found in the following collections Email alerting service here right-hand corner of the article or click Receive free email alerts when new articles cite this article-sign up in the box at the top cerevisiae W303-K6001 cross-platform genome sequence: insights into ancestry and physiology of a laboratory mutt. The Saccharomyces cerevisiae W303-K6001 cross-platform genome sequence: insights into ancestry and physiology of a laboratory mutt 1. Summary Saccharomyces cerevisiae strain W303 is a widely used model organism. However, little is known about its genetic origins, as it was created in the 1970s from crossing yeast strains of uncertain genealogy. To obtain insights into its ancestry and physiology, we sequenced the genome of its variant W303-K6001, a yeast model of ageing research. The combination of two next-generation sequencing (NGS) technologies (Illumina and Roche/454 sequencing) yielded an 11.8 Mb genome assembly at an N50 contig length of 262 kb. Although sequencing was substantially more precise and sensitive than whole-genome tiling arrays, both NGS platforms produced a number of false positives. At a 378Â average coverage, only 74 per cent of called differences to the S288c reference genome were confirmed by both techniques. The consensus W303-K6001 genome differs in 8133 positions from S288c, predicting altered amino acid sequence in 799 proteins, including factors of ageing and stress resistance. The W303-K6001 (85.4%) genome is virtually identical (less than equal to 0.5 variations per kb) to S288c, and thus originates in the same ancestor. Non-S288c regions distribute unequally over the genome, with chromosome XVI the most (99.6%) and chromosome XI the least (54.5%) S288c-like. Several of these clusters are shared with S1278B, another widely used S288c-related model, indicating that these strains share a second ancestor. Thus, the W303-K6001 genome pictures details of complex genetic relationships between the model strains that date back to the early days of experimental yeast genetics. Moreover, this study underlines the necessity of combining multiple NGS and genome-assembling techniques for achieving accurate variant calling in genomic studies.


Introduction
The small heat-shock protein (sHSP) class of protein chaperone is an energyindependent class of chaperone that act as holdases when combating the potential of misfolded or damaged proteins to form aggregates. sHSPs are also assembly chaperones, associating with protein polymers, such as actin, microtubules and intermediate filaments to modulate both their oligomerization and their assemblies in cells. How such properties relate to the structure of sHSP oligomers is a key question.
HSP16.5 from Methanocaldococcus jannaschii (MjHSP16.5) was the first sHSP to be structurally characterized by crystallography [1]. The protein oligomerizes into a particle containing 24 identical subunits with octahedral symmetry. The crystal structure confirmed earlier protein structure predictions [2] regarding a central a-crystallin domain (ACD) forming a b-strand sandwich comprised eight b-strands (numbered 2-9) organized in an immunoglobulin-like fold that is flanked by the N-and C-terminal domains that dock onto hydrophobic sites on the b4/b8 edge of this b-strand sandwich at the fourfold symmetry axis of the oligomer. The b6-strand hydrogen bonds to the b2-strand of its partner subunit in the twofold axis, forming an inter-subunit composite b-sheet, a feature found in the crystal structures of other non-metazoan sHSP oligomers [1,3,4]. The b6-strand therefore contributes to the oligomerization of individual sHSPs [5][6][7]. The edge formed by the b2-and b7-strands is where one of the highly conserved arginine residues [8] locates: precisely, the one which when mutated in aB-crystallin (CRYAB, CTPP2, HSPB5; [9]) and aA-crystallin (CRYAA, CRYA1, HSPB4; [10]), causes cataract (R116C CRYAA and R120G CRYAB) and cardiomyopathy (R120G CRYAB).
Previous studies on the R120G mutation in CRYAB revealed an increase in oligomer size [11]. Moreover, the R120G CRYAB oligomer had lost both its temperature and pressure dependence for size, and was also compromised in its heat stability compared with wild-type (WT) CRYAB [11]. By comparing a series of substitutions at the R120 site, it was apparent that the dimer, via its intra-and inter-subunit interactions, was very important to oligomerization and particle size [11]. Furthermore, for MjHSP16.5, oligomerization and particle size can also be altered by inserting extra sequences in its N-terminal domain as seen for HSP16.5-P1N [12].
Oligomerization differs remarkably between metazoan and non-metazoan orthologues. Non-metazoan sHSPs classically form oligomers of a defined subunit number, but metazoan sHSP complexes are typically polydisperse [8,13]. In non-metazoan sHSPs, the b6-strand is integrated into its partner dimer subunit, but this does not occur in metazoan sHSPs because the loop separating the b6-and b7-strands is non-existent, and they are instead fused into one [14]. One highly conserved structural element within the sHSP family [2,8,13] that is critical to oligomerization and to sHSP polydispersity is the C-terminal domain and specifically the IXI motif, which because of its palindromic nature, allows two different binding modes into the groove of the b4/b8 edge of the ACD [15]. The position and structural function of the IXI motif was identified in the crystal structures of MjHSP16.5 [12,16] as well as in the crystal structure of wheat HSP16.9 [4]. The MjHSP16.5 IXI motif and C-terminal extension therefore links together the four and threefold units in the oligomer, and its deletion would be expected to be deleterious for oligomerization [16]. This notion is further supported by the findings that deletion of the IXI motif prevents oligomerization of both bacterial and plant sHSPs [17,18]. The mammalian sHSP, HSPB6, is naturally missing this motif and does not oligomerize, forming dimers instead [19]. For a mammalian sHSP that does possess an IXI motif, deletion of C-terminal sequences including the IXI motif in CRYAB (Q151X in CRYAB) not only prevents its selfoligomerization, but also destabilizes it, with the benefit of apparently increasing its binding to client proteins [20]. Mutating the IXI to G-X-G in either CRYAB or CRYAA did not prevent oligomerization, but did improve its function in client protein protection assays [21]. Indeed, solution nuclear magnetic resonance (NMR) studies have shown that the flexibility of the IXI motif, and the C-terminal extension, in general, is critical to its function and point mutations that alter this flexibility change the activity of the sHSP [22]. Therefore, it is clear that this motif is key to sHSP protein oligomerization, dynamics and function, and it is important to formally confirm that this key role for the IXI motif is preserved in MjHSP16. 5.
In this study, we analyse the effect of deleting the IXI motif and substitute the highly conserved arginine (R107) equivalent to the disease-causing mutation in CRYAB (R120G) into MjHSP16.5. In addition, we present the crystal structure for R107G MjHSP16.5 and by cryo-electron microscopy document a temperature-dependent effect upon its quaternary structure and assess the functional consequences by in vitro chaperone assays. The expression constructs of both WT and engineered (R107G, G143X) MjHSP16.5 were transformed into BL21(DE3) pLysS Escherichia coli. Recombinant protein expression was induced using 0.5 mM IPTG for 4 h, once the bacterial culture had reached an OD 600 of 0.6. Harvested pellets were resuspended in 50 mM Tris-HCl pH 8, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.2 mM PMSF containing complete protease inhibitor cocktail (Roche) and lysed by several freeze/thaw cycles. A supernatant fraction containing the soluble MjHSP16.5 was prepared by centrifugation of the lysate at 15 000 rpm in a JA-20 rotor (Beckman) for 30 min at 48C after incubation for 30 min with 10 U ml 21 of benzonase nuclease (Merck Bioscience) at 48C followed by 0.005% (w/v) polyethyleneimine for 10 min on ice.

(b) Purification of MjHSP16.5 proteins
Methanocaldococcus jannaschii is a hyperthermophile and lives in hyperthermal oceanic vents, which experience temperatures of approximately 50 -958C and NaCl concentrations of approximately 0.2-1.0 M [23]. These organisms must therefore possess unique adaptations to allow survival in such high temperatures and salinity levels [24]. With this as a background, the soluble protein fraction was incubated in a water bath at 808C for 30 min in the presence of 1 M NaCl. Samples were then cooled on ice for 10 min and centrifuged at 48 000g for 30 min. By using this approach, almost all contaminating proteins are denatured and pelleted, and the resulting soluble fraction was 90 -95% MjHSP16.5. The protein was then further purified by rstb.royalsocietypublishing.org Phil Trans R Soc B 368: 20120327 anion exchange chromatography and size exclusion chromatography (SEC). Purification by anion exchange chromatography used a Fractogel EMD-650S TMAE column. Peak fractions were pooled and then dialysed into 20 mM Tris-HCl pH 7.4, 100 mM NaCl in preparation for SEC.
The R107G and G143X MjHSP16.5 proteins were purified using the same purification methods as WT MjHSP16.5, by heating and centrifugation, anion exchange and SEC. Purity was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry as described [20]. For G143X MjHSP16.5, it was difficult to achieve more than 90 per cent. Although G143X MjHSP16.5 was heat stable, crystallization was not attempted. All pure protein samples were analysed by electron spray ionization mass spectrometry, and the measured molecular weights were within 2 kDa of the calculated molecular weight (data not shown).

(c) Size exclusion chromatography of small heat-shock proteins
To estimate the molecular size of the recombinant sHSP complexes, approximately 5 mg of purified proteins were subjected to SEC. Purified MjHSP16.5 proteins were analysed using a Superose 6 column (290 Â 10 mm) at a flow rate of 0.2 ml per minute at room temperature using a Merck-Hitachi biochromatography system. The column was calibrated with the following molecular mass standards: thyroglobulin (669 kDa), apoferritin (440 kDa), b-amylase (200 kDa), bovine serum albumin (67 kDa) and carbonic anhydrase (29 kDa). The void volume of the column was determined by dextran blue (2000 kDa). The proteins were separated on the column in buffer containing either 20 mM Tris-HCl, pH 7.4 or 10 mM HEPES, pH 7.4 and 100 mM NaCl at room temperature, and the elution volume of each sample was used to estimate the molecular weight. The data were analysed using CHROMELEON 6.30 software (Sunnyvale, CA, USA).

(d) Chaperone assays
Citrate synthase from porcine heart (Sigma-Aldrich, UK) was dialysed for 16 h at 48C against 50 mM Tris-HCl pH 8.0, 2 mM EDTA and diluted to 0.49 mg ml 21 . The assay mixture contained citrate synthase and MjHSP16.5 at a substrate : chaperone (4 : 1) ratio in 220 ml at a temperature of 428C. The effectiveness of MjHSP16.5 as a chaperone at 608C was measured using b L -crystallin as the client protein [25] and a-crystallin as a positive control. Both a-crystallin and b L -crystallin from bovine lens (Sigma-Aldrich) were dissolved in 50 mM sodium phosphate (pH 7.4), 100 mM NaCl. A chaperone-to-client ratio (1 : 1) by weight was used. Time-dependent light scattering was measured at 360 nm every 15 s over 30 min, using a Beckman DU640 spectrophotometer for both chaperone assays.

(e) Crystallization
Initial crystallization experiments were performed using an Innovadyne screenmaker and standard sitting drop trays with drop sizes of 100 and 200 nl protein solution, respectively, mixed with 100 nl reservoir solution. Small crystals were observed in a number of conditions containing 2-methyl-2,4-pentanediol (MPD) and low molecular weight polyethylene glycol. The largest, best diffracting crystals were obtained from follow-up trays with hanging drop geometry using a reservoir solution of 0.02 M sodium acetate pH 5.5, 0.02 M CalCl 2 and 35 per cent MPD as precipitating agents. Crystals grew over a period of weeks to maximum dimensions of 1.0 Â 0.5 Â 0.5 mm.
(f ) Data collection, processing and structure determination Crystals were typically tested using the in-house Bruker Micro-Star rotating anode equipped with a platinum 135 CCD detector [26]. All final diffractions were collected at the Diamond beam line I02 [27] and processed with XDS [28]. The structures of the WT and the R107G variant were solved by molecular replacement using the original MjHSP16.5 structure [1] with AMoRe [29], and refined using REFMAC [30]. All interactive modelling was performed with Coot [31]. Further details of the crystal structure determinations are summarized in table 1. Coordinates and structure factors for the R107G MhHSO16.5 have been deposited at the protein data bank (PDB ID: 4I88). (g) Cryo-electron microscopy The final concentrations of both R107G and WT MjHSP16.5 used for cryo-electron microscopy (cryoEM) study were 0.8 mg ml 21 . The sample (3.5 -4.5 ml) was applied to the GIG holey grids (LifeTrust, PR China) after the girds were treated with a glow-discharge machine (Master Plasmer). Then, the samples were blotted for 3 s with blot force three at 100 per cent humidity using the VitrobotMark IV (FEI, The Netherland). The temperature of the Vitrobot chamber was set to room temperature and 608C, respectively, for both the WT and the R107G MjHSP16.5 proteins. The grids were fast frozen into liquid-nitrogen-cooled liquid ethane.
The frozen samples were imaged with a FEI 300 kV Titan Krios cryo-electron microscope equipped with a Gatan Ultra-Scan4000 (model 895) 16-megapixel CCD. A nominal magnification of 96 000Â corresponding to the pixel size 0.933 Å and a defocus range between 22 and 23 mm were used during data collection for the WT MjHSP16.5 both at room temperature and at 608C and for R107G MjHSP16.5 at 608C. For the mutant R107G at room temperature, a nominal magnification of 75 000Â was used, which corresponds to the pixel size 1.196 Å . The electron dose per image was kept around 20 e 2 Å 22 . For the R107G MjHSP16.5 at room temperature, 126 raw CCD electron micrographs were collected manually. For R107G MjHSP16.5 at 608C and the WT MjHSP16.5 at both room temperature and at 608C, electron micrograph exposures were made with the automatic collection package Leginon [33]. In total, 1308 raw images were collected for the R107G MJHSP16.5 at 608C, 1160 raw images for the WT at room temperature and 2119 raw images for the WT MjHSP16.5 at 608C. The EM maps for both WT and R107G HSP16. 5

(h) Image processing
The defocus and astigmatism of each micrograph were estimated with CTFFIND3 [34] and corrected using the 'applyctf' routine of EMAN1 [35]. Particles of R107G (14 702 and 12 870) at room temperature and at 608C and particles of the WT MjHSP16.5 (21 001 and 38 220) at room temperature and at 608C were picked out using a graphical processing unit-accelerated, templatebased, particles selection program, GAUTOMATCH, developed in Fei Sun's laboratory (to be published). The initial models for the earlier-mentioned four different samples were obtained by using 'startoct' in EMAN1 [35]. Two different levels of noises were added into each initial model to yield two additional models. Then for each sample, these three corresponding models were used for further competitive image alignment and model refinement by using 'multirefine' in EMAN1 [35]. During the three-dimensional reconstruction, the octahedron symmetry was imposed. The final reconstructed density map was further sharpened by application of an amplitude correction algorithm in the program BFACTOR [36]. CryoEM maps were segmented, displayed and fitted with atomic models, using UCSF Chimera [37]. The crystal structure of the WT MjHSP16.5 (PDB code 1SHS) was used to fit into the cryoEM maps using the UCSF Chimera package [37].
The diameters of the reconstructed HSP16.5 particles were measured using the rotation-averaged projection method. First, the reconstructed volumes were projected by the same azimuthal angle to get the corresponding projections. Second, the projections were rotation-averaged using 'proc2d' in EMAN1 [35]. Finally, the rotational-averaged projections were projected along the y-direction to obtain the respective one-dimensional profiles. The distance between the two minimal values of the one-dimensional profile was measured as the diameter of the reconstructed particle.

Results
(a) Characterization of wild-type, R107G and G143X MjHSP16.5 Wild-type MjHSP16.5 and the two engineered proteins R107G (the G107 substitution) and G143X MjHSP16.5 (the IXI motif deletion construct) were produced recombinantly in E. coli ( figure 1a). The WT and R107G MjHSP16.5 were both purified using the modified protocol, which included a heating step (808C for 30 min in the presence of 1M NaCl) to remove as many E. coli proteins as possible. Purification was completed by a final SEC step. Although it was previously found that at 608 8 8 8 8C the protein tended to aggregate [38], this was not our experience (see below). R107G MjHSP16.5 was purified similarly, but the same degree of purification for G143X MjHSP16. (b) Crystal structures of R107G MjHSP16.5 and comparison with the wild-type structure The R107G MjHSP16.5 crystallizes in the same rhombohedral space group with eight independent molecules per asymmetric unit as the WT protein [1]. The functional 24mer generated by crystallographic threefold axis is shown in ribbon representations in three different orientations ( figure 2). Owing to the high diffraction data quality, the mutation is unambiguously confirmed even in the initial electron density map calculated with the molecular replacement solution using the WT structure. The negative electron density on all eight independent protein chains confirms the absence of side chain atoms ( figure 3a). Clearly, the mutation does not cause any significant change in oligomeric state and overall structure (figure 3b).

rstb.royalsocietypublishing.org Phil Trans R Soc B 368: 20120327
Closer comparison between the higher resolution WT structure presented here and the R107G MjHSP16.5 shows that there are no significant changes in the main chain positions (figure 3b). The root-mean-square deviation of 6299 equivalent main chain atoms is only 0.45 Å -clearly within the accuracy of the crystal structure determination. Hence, even locally, there are no significant structural changes detectable. However, it is noteworthy that the R107 in WT MjHSP16.5 forms hydrogen bonds to the main chain carbonyl oxygen atoms of G41 and M43, and forms electrostatic interactions with the residue E98 of the partner chain (figure 3c). Although the R107G substitution does not affect the overall crystal structure of the protein in the crystal environment, the loss of these two H-bonds and the inter-molecular electrostatic interaction in the R107G MjHSP16.5 variant may very well change the dynamic behaviour in solution. (c) Cryo-electron microscopy reconstruction of wild-type and R107G MjHSP16.5 at room temperature and at 608C The WT and R107G MjHSP16.5 incubated at room temperature and at 608C were imaged and analysed by cryo-electron microscopic single particle reconstruction (see figure 4 and electronic supplementary material, figures S1 and S2). The   ) and the other has a closed structure at the same region (see figure 4f and electronic supplementary material, figure S1b). The chamber of both structures for R107G MjHSP16.5 at room temperature contains electron density. The final reconstructions of R107G MjHSP16.5 at 608C, however, revealed two significantly different structures with distinct diameters (see figure 4g,h and electronic supplementary material, figure S1c,d). The two different diameters of R107G MjHSP16.5 particles at 608C were obvious after two-dimensional reference free classification (see the electronic supplementary material, figure S2). The smaller of the two particles is similar in size (diameter 12.4 nm) to the R107G and WT MjHSP16.5 particles at room temperature. Interestingly, the central chamber of this smaller (12.4 nm) particle for R107G MjHSP16.5 is empty in contrast to similar-sized particles at room temperature. The larger of the two, however, has a significantly increased diameter of 13.6 nm and was larger than any other structure derived in this study. These two reconstructions of the particles revealed different oligomer arrangements in the fourfold symmetry axis and the dimer -dimer interface for R107G MjHSP16.5 oligomers (see figure 5 and electronic supplementary material, figure S3). The dimer -dimer interface within the 12.4 nm diameter particle is equivalent to the arrangement in the crystal structures of WT (see the electronic supplementary material, figure S3) and R107G MjHSP16.5 (figure 5a,e). However, the distance between the dimers in the 13.6 nm particle is increased to reveal a large space at the dimer -dimer interface ( figure 5d,f ).

(d) The R107G mutation alters the chaperone activity of
MjHSP16.5 at 608C The cryo-electron microscopy data suggest that the R107G substitution allows for some oligomer flexibility and therefore we considered whether this could influence the in vitro chaperone activity of MjHSP16.5. For this purpose, we used one of the earliest assays used to demonstrate the chaperone activity of sHSPs [25] because it could be conducted at 608C and so be consistent with the cryo-electron microscopy analyses. As previously reported [25], a-crystallin very effectively prevented the heat-induced aggregation of b L -crystallin (figure 6a) when present in a 1 : 1 ratio (figure 6b). Similarly, R107G MjHSP16.5 was equally effective in suppressing the heat-induced aggregation of this artificial client protein. This is in stark contrast to the WT MjHSP16.5 (figure 6a), which appeared ineffective. Under the conditions of this assay and in the absence of an effective chaperone, b L -crystallin formed aggregates that over the course of the assay were large enough to settle under gravity, which is the reason for the variable signal towards the end of the assay. Clearly, the effect of the addition of MjHSP16.5 was quite distinct from that of either a-crystallin or R107G MjHSP16.5.

Discussion
(a) The role of the N-and C-terminal domains on oligomerization of MjHSP16.5 Here, we have shown that removal of the C-terminal 5 residues from MjHSP16.5 is sufficient to significantly alter its oligomerization and also its chaperone activity towards citrate synthase (figure 1e). Our data are consistent with  previous reports and with a key role for this domain in the oligomerization of MjHSP16.5 [16]. Preventing oligomerization by the removal of C-terminal residues mimic data obtained for CRYAB, where the myopathy-causing mutation Q151X in CRYAB prevented subunit oligomerization, but also increased its activity in in vitro chaperone assays [20]. Similar results are presented for MjHSP16.5. Altering the N-terminal sequences in MjHSP16.5 can also increase the particle size with subunit number increased from 24 to 48 and also the client-protein-binding properties of the protein complex [12]. Swapping the N-terminal domain of CRYAB with that from CRYAA had a similar remarkable effect upon oligomerization and upon its chaperone function [39]. Similarly, deletion of the N-terminal domain prevents the oligomerization of mammalian sHSPs, for instance, CRYAB [15], and solid-state NMR studies of CRYAB demonstrate that this domain, which includes the b2-strand, interacts with the b3-strand of a neighbouring dimer [40]. Indeed, removing both the N-and C-terminal domains of CRYAB produces a stable ACD dimer that can be crystallized [14,15]. Therefore, it is clear that though there is significant sequence variation in the N-and C-terminal sequences between members of the sHSP family, they influence greatly sHSP subunit oligomerization and also their chaperone function [41].
(b) Influence of the R107G mutation on the dimer interface and MjHSP16.5 oligomerization Recent data suggest that the origin of metazoan sHSP particle size heterogeneity resides in the protein dimer and specifically the ACD [42]. The CRYAB ACD dimer can adopt different conformations as revealed by crystallography [14,15] and by solid-state NMR studies [40]. Mutations in the ACD that alter the dimer conformation would be expected to significantly change sHSP oligomerization, and one example of this is the disease-causing mutation R120G in CRYAB [42]. R120 in CRYAB is part of an extensive ionic interaction network that crosses the dimer interface and it influences the oligomerization properties of both CRYAB and CRYAA [43,44]. This arginine is a very highly conserved residue within the ACD domain of sHSPs [8].
As shown in this study, the introduction of glycine instead of arginine at position 107 had several interesting structural consequences for MjHSP16.5. In the first instance, the crystal structures revealed no significant difference in the electron density maps for the crystals of WT or R107G MjHSP16.5. This is likely due to the fact that in non-metazoans, the b6-strand exchanges between partner chains, which is absent in metazoan sHSPs [14,15,45], together with the crystallization conditions that constrained the oligomer packing. It was only by cryo-electron microscopy that structural differences around the dimer interface at the fourfold axis were observed and then only for MjHSP16.5 heated to 608C. The WT MjHSP16.5 had two distinct classes represented in the samples prepared at room temperature and at 608C, which is consistent with previous studies [38]. No changes in the dimer subunit arrangements were observable for these two different-sized particles. Indeed, the difference in these two particles was seen similarly for the R107G MjHSP16.5 at room temperature (figure 4). In both cases, the subunit compliment was unchanged (see figure 5 and electronic supplementary material, figure S3).
The dimer-dimer interface within the 12.4 nm diameter particle is equivalent to the arrangement in the crystal structures of WT (see the electronic supplementary material, figure S3) and R107G MjHSP16.5 (figure 5). The distance between the dimers in the 13.6 nm particle is increased to reveal a large space at the dimer-dimer interface. Each monomer has to adapt the conformation of its ACD loop to make appropriate interactions with the adjacent dimer. Such conformational change can be easily achieved as a result of the R107 substitution with glycine because this would abrogate the electrostatic interaction in the dimer between R107 in one monomer and E98 in an adjacent subunit to allow more flexibility in the ACD loop (figure 5), and this most likely is the reason why changes in the dimer interface can be accommodated without changing the subunit number in the assembled particle. This interpretation is consistent with the view that inter-subunit interactions are very important to oligomer size [11,42].
(c) Influence of the R107G mutation on the chaperone function of MjHSP16.5 The R107G substitution in MjHSP16.5 also has functional consequences as evidenced by the increased chaperone activity towards b L -crystallin at 608C ( figure 6). The R120G substitution in CRYAB also changed its chaperone activity, either ablating completely or reducing it for the client proteins tested [43,44,[46][47][48]. In CRYAB, there is an extensive network of ionic interactions that determine the AP interface between dimers [15], and these are disrupted by the R120G mutation in CRYAB causing the groove formed at the dimer interface to narrow [42]. This groove is also where small peptides bind in CRYAB [42]. If it is assumed that the equivalent interface in MjHSP16.5 can also potentially bind to client, then because this interface adopts a more open state at 608C this would offer a potential explanation of its improved chaperone function. Thermal-induced plasticity in chaperone activity combined with oligomerization has been observed for pea HSP18.1 [49]. HSP18.1 is a monodisperse dodecamer at ambient (228C) temperatures, but heat shock (388C) causes higher-order oligomeric states to form [49]. The addition of unfolding client proteins induces the formation of HSP18.1-client protein complexes that are remarkably polydisperse, varying in both the number of sHSP subunits and client proteins. WT and R107G MjHSP16.5 are constrained in their oligomerization dispersity. Nevertheless, the cryo-electron microscopy data indicate that R107G MjHSP16.5 forms at least two particles of different diameter, but of constant subunit number. Others have reported that WT MjHSP16.5 is also structurally diverse for the 24mer [38,50] and our data reveal two 12.4 nm particles differing in central density. The chaperone activity of MjHSP16.5 can also be increased when oligomer dispersity is driven by N-terminal sequence changes [12,16]. In the case of MjHSP16.5-P1 [16], there was no change in either the subunit number, the dimer interface or the symmetry, rather only a change in the orientation of the C-terminal sequences. This led to the suggestion that shell size for MjHSP16.5 depends on how the N-terminal sequences influence the C-terminal sequence orientation in MjHSP16.5-P1 [16]. The observed increase in client protein affinity and chaperone activity was reasoned to be due to the exposure of the N-terminal sequences [41,51]. We document here that preventing the rstb.royalsocietypublishing.org Phil Trans R Soc B 368: 20120327 oligomerization of MjHSP16.5 by deleting the C-terminal five residues increases in vitro chaperone activity, which parallels data obtained for CRYAB [20]. While our data do not distinguish between the two current models of sHSP-client protein recognition and binding [51,52], the increased chaperone activity towards b L -crystallin at 608C as a result of the R107G substitution in MjHSP16.5 illustrates how such substitutions within the ACD can also contribute to structural and functional diversity of MjHSP16.5.

Note added in proof
During the review of this manuscript, Shi et al. [53] reported cryo-electron microscope analysis for both WT and R107 MjHSP16.5. This study reported differences in the density distribution with the central cavity, which were interpreted to be due to changes in the relative position of the N-terminal domain as a result of the R107G substitution and indicating that R107G MjHSP16.5 was in an activated state. The data presented here are consistent with this interpretation, but only when the data for reconstructed particles at 608C are considered. Moreover, our data demonstrate that the R107G substitution also facilitates the expansion of the ACD shell without the necessity of additional subunits.